TRIP - Transgenic Rats ImmunoPhenomic

Scientific head

Technical participants

Laurent Tesson
Research Assistant
Laure-Hélène Ouisse
Research Assistant
Vanessa Quillaud-Chenouard
Research Assistant

TRIP platform has been created in 1996 and has ever since developed Knock-Out (KO) or Knock-In (KI) rat research models for all (academic or private labs, French or international companies…)

  • Gene or large genomic locus function analysis: Gene(s) KO
  • SNP studies: KI to induce mutation of interest
  • Cellular or protein tracking: reporter KI in frame with your marker protein
  • Protein or reporter gene overexpression: KI of your protein of interest or reporter under the control of ubiquitous promoter
  • Spatio-temporal control by conditional models
  • Human cells or tissues engraftment: immunodeficient models

To discuss your own project or if you are interested in our available research models, please contact Ignacio Anegon

AVAILABLE RESEARCH MODELS

Reporter GFP rats (alive & cryoconserved)

Remy, S. et al. (2010). New lines of GFP transgenic rats relevant for regenerative medicine and gene therapy. Transgenic Research19(5), 745‑763. https://doi.org/10.1007/s11248-009-9352-2

 

Immunodeficient rats

  • RRG KO (rIL2Rg KO / rRag-1 KO) (cryoconserved)Ménoret, S., et al. (2018). Generation of Immunodeficient Rats With Rag1 and Il2rg Gene Deletions and Human Tissue Grafting Models. Transplantation102(8), 1271‑1278. https://doi.org/10.1097/TP.0000000000002251
  • RRGS (RRG KO with human SIRPα) (alive & cryoconserved)Ménoret, S., et al. 2019. « In Vivo Analysis of Human Immune Responses in Immunodeficient Rats ». Transplantation, novembre. https://doi.org/10.1097/TP.0000000000003047

 

Duchenne Muscular Dystrophy  (DMD) disease rat model (alive & cryoconserved)

Larcher, T., Lafoux, A., Tesson, L., Remy, S., Thepenier, V., François, V., … Huchet, C. (2014). Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy. PloS One9(10), e110371. https://doi.org/10.1371/journal.pone.0110371

Ouisse, Laure-Hélène, Séverine Remy, Aude Lafoux, Thibaut Larcher, Laurent Tesson, Vanessa Chenouard, Carole Guillonneau, et al. 2019. « Immunophenotype of a Rat Model of Duchenne’s Disease and Demonstration of Improved Muscle Strength After Anti-CD45RC Antibody Treatment ». Frontiers in Immunology 10 (septembre): 2131. https://doi.org/10.3389/fimmu.2019.02131

Caudal, Dorian, Virginie François, Aude Lafoux, Mireille Ledevin, Ignacio Anegon, Caroline Le Guiner, Thibaut Larcher, et Corinne Huchet. 2020. « Characterization of brain dystrophins absence and impact in dystrophin-deficient Dmdmdx rat model ». PLoS ONE 15 (3). https://doi.org/10.1371/journal.pone.0230083

 

Other reporter and conditional models are available in repositories and vendors

 

PARTNERS

 

PUBLICATIONS

2020

  • Menchaca, A., Dos Santos-Neto, P. C., Souza-Neves, M., Cuadro, F., Mulet, A. P., Tesson, L., Chenouard, V., Guiffès, A., Heslan, J. M., Gantier, M., Anegón, I., & Crispo, M. (2020). Otoferlin gene editing in sheep via CRISPR-assisted ssODN-mediated Homology Directed Repair. Scientific Reports, 10(1), 5995. https://doi.org/10.1038/s41598-020-62879-y
  • Ménoret, S., Ouisse, L.-H., Tesson, L., Remy, S., Usal, C., Guiffes, A., Chenouard, V., Royer, P.-J., Evanno, G., Vanhove, B., Piaggio, E., & Anegon, I. (2020). In Vivo Analysis of Human Immune Responses in Immunodeficient Rats. Transplantation, 104(4), 715–723. https://doi.org/10.1097/TP.0000000000003047
  • Manaud, G., Nossent, E. J., Lambert, M., Ghigna, M.-R., Boët, A., Vinhas, M.-C., Ranchoux, B., Dumas, S. J., Courboulin, A., Girerd, B., Soubrier, F., Bignard, J., Claude, O., Lecerf, F., Hautefort, A., Florio, M., Sun, B., Nadaud, S., Verleden, S. E., … Perros, F. (2020). Comparison of Human and Experimental Pulmonary Veno-Occlusive Disease. American Journal of Respiratory Cell and Molecular Biology. https://doi.org/10.1165/rcmb.2019-0015OC
  • Caudal, D., François, V., Lafoux, A., Ledevin, M., Anegon, I., Le Guiner, C., Larcher, T., & Huchet, C. (2020). Characterization of brain dystrophins absence and impact in dystrophin-deficient Dmdmdx rat model. PLoS ONE, 15(3). https://doi.org/10.1371/journal.pone.0230083
  • Adigbli, G., Ménoret, S., Cross, A. R., Hester, J., Issa, F., & Anegon, I. (2020). Humanization of Immunodeficient Animals for the Modeling of Transplantation, Graft Versus Host Disease and Regenerative Medicine. Transplantation. https://doi.org/10.1097/TP.0000000000003177
  • Fourrier, A., Delbos, F., Menoret, S., Collet, C., Thi Thuy, L. T., Myara, A., Petit, F., Tolosa, L., Laplanche, S., Gómez-Lechón, M. J., Labrune, P., Anegon, I., Vallier, L., Garnier, D., & Nguyen, T. H. (2020). Regenerative cell therapy for the treatment of hyperbilirubinemic Gunn rats with fresh and frozen human induced pluripotent stem cells-derived hepatic stem cells. Xenotransplantation, 27(1), e12544. https://doi.org/10.1111/xen.12544

2019

  • Kenawi, Moussa, Emmanuel Rouger, Marie-Laure Island, Patricia Leroyer, François Robin, Séverine Rémy, Laurent Tesson, et al. (2019). Ceruloplasmin Deficiency Does Not Induce Macrophagic Iron Overload: Lessons from a New Rat Model of Hereditary Aceruloplasminemia. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology, septembre, fj201901106R. https://doi.org/10.1096/fj.201901106R
  • Lambert, Mélanie, Véronique Capuano, Angèle Boet, Laurent Tesson, Thomas Bertero, Morad K. Nakhleh, Séverine Remy, et al. (2019). Characterization of Kcnk3-Mutated Rat, a Novel Model of Pulmonary Hypertension. Circulation Research 125 (7): 678‑95. https://doi.org/10.1161/CIRCRESAHA.119.314793
  • Ouisse, Laure-Hélène, Séverine Remy, Aude Lafoux, Thibaut Larcher, Laurent Tesson, Vanessa Chenouard, Carole Guillonneau, et al. (2019). Immunophenotype of a Rat Model of Duchenne’s Disease and Demonstration of Improved Muscle Strength After Anti-CD45RC Antibody Treatment. Frontiers in Immunology 10: 2131. https://doi.org/10.3389/fimmu.2019.02131

2018

  • Clarke, Starlynn C., Biao Ma, Nathan D. Trinklein, Ute Schellenberger, Michael J. Osborn, Laure-Hélène Ouisse, Andrew Boudreau, et al. (2018). Multispecific Antibody Development Platform Based on Human Heavy Chain Antibodies. Frontiers in Immunology 9: 3037. https://doi.org/10.3389/fimmu.2018.03037
  • Charpentier, M., Khedher, A. H. Y., Menoret, S., Brion, A., Lamribet, K., Dardillac, E., … Concordet, J. P. (2018). CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Nature Communications, 9(1), 1133. https://doi.org/10.1038/s41467-018-03475-7
  • Ménoret, S., Ouisse, L.-H., Tesson, L., Delbos, F., Garnier, D., Remy, S., … Anegon, I. (2018). Generation of Immunodeficient Rats With Rag1 and Il2rg Gene Deletions and Human Tissue Grafting Models. Transplantation, 102(8), 1271‑1278. https://doi.org/10.1097/TP.0000000000002251
  • Ossart, J., Moreau, A., Autrusseau, E., Ménoret, S., Martin, J. C., Besnard, M., … Guillonneau, C. (2018). Breakdown of Immune Tolerance in AIRE-Deficient Rats Induces a Severe Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy-like Autoimmune Disease. Journal of Immunology (Baltimore, Md.: 1950), 201(3), 874‑887. https://doi.org/10.4049/jimmunol.1701318
  • Ouisse, L.-H., Remy, S., Lafoux, A., Larcher, T., Tesson, L., Chenouard, V., … Anegon, I. (2018). Immunological characterization of a rat model of Duchenne’s disease and demonstration of improved muscle strength after anti-CD45RC antibody treatment. BioRxiv, 407023. https://doi.org/10.1101/407023

2017

  • Lopez Robles, M. D., Pallier, A., Huchet, V., Le Texier, L., Remy, S., Braudeau, C., … Chiffoleau, E. (2017). Cell-surface C-type lectin-like receptor CLEC-1 dampens dendritic cell activation and downstream Th17 responses. Blood Advances, 1(9), 557‑568. https://doi.org/10.1182/bloodadvances.2016002360
  • Ménoret, S., Tesson, L., Remy, S., Usal, C., Ouisse, L.-H., Brusselle, L., … Anegon, I. (2017). Advances in transgenic animal models and techniques. Transgenic Research, 26(5), 703‑708. https://doi.org/10.1007/s11248-017-0038-x
  • Ouisse, L.-H., Gautreau-Rolland, L., Devilder, M.-C., Osborn, M., Moyon, M., Visentin, J., … Saulquin, X. (2017). Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies. BMC Biotechnology, 17(1), 3. https://doi.org/10.1186/s12896-016-0322-5
  • Remy, S., Chenouard, V., Tesson, L., Usal, C., Ménoret, S., Brusselle, L., … Anegon, I. (2017). Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation. Scientific Reports, 7(1), 16554. https://doi.org/10.1038/s41598-017-16328-y

2016

  • Chenouard, V., Brusselle, L., Heslan, J.-M., Remy, S., Ménoret, S., Usal, C., … Tesson, L. (2016). A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals: A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 43(5), 341‑348. https://doi.org/10.1016/j.jgg.2016.04.005
  • Fontaniere, S., Braun V, Ménoret S, Anegon I, Merrouche C, Fraichard A, & Cherifi Y. (2016). Pronuclear injection for the production of transgenic rats. Cold Spring Harbor protocols.
  • Jung, C. J., Ménoret, S., Brusselle, L., Tesson, L., Usal, C., Chenouard, V., … Anegon, I. (2016). Comparative Analysis of piggyBac, CRISPR/Cas9 and TALEN Mediated BAC Transgenesis in the Zygote for the Generation of Humanized SIRPA Rats. Scientific Reports, 6, 31455. https://doi.org/10.1038/srep31455
  • Martin, J. C., Bériou, G., Heslan, M., Bossard, C., Jarry, A., Abidi, A., … Josien, R. (2016). IL-22BP is produced by eosinophils in human gut and blocks IL-22 protective actions during colitis. Mucosal Immunology, 9(2), 539‑549. https://doi.org/10.1038/mi.2015.83
  • Menchaca, A., Anegon, I., Whitelaw, C. B. A., Baldassarre, H., & Crispo, M. (2016). New insights and current tools for genetically engineered (GE) sheep and goats. Theriogenology, 86(1), 160‑169. https://doi.org/10.1016/j.theriogenology.2016.04.028
  • Renaud, J.-B., Boix, C., Charpentier, M., De Cian, A., Cochennec, J., Duvernois-Berthet, E., … Giovannangeli, C. (2016). Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases. Cell Reports, 14(9), 2263‑2272. https://doi.org/10.1016/j.celrep.2016.02.018
  • Tesson, L., Remy, S., Ménoret, S., Usal, C., Thinard, R., Savignard, C., … Anegon, I. (2016). Genome Editing in Rats Using TALE Nucleases. Methods in Molecular Biology (Clifton, N.J.), 1338, 245‑259. https://doi.org/10.1007/978-1-4939-2932-0_18

2015

  • Crispo, M., Mulet, A. P., Tesson, L., Barrera, N., Cuadro, F., dos Santos-Neto, P. C., … Menchaca, A. (2015). Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes. PloS One, 10(8), e0136690. https://doi.org/10.1371/journal.pone.0136690
  • Durand, J., Huchet, V., Merieau, E., Usal, C., Chesneau, M., Remy, S., … Chiffoleau, E. (2015). Regulatory B Cells with a Partial Defect in CD40 Signaling and Overexpressing Granzyme B Transfer Allograft Tolerance in Rodents. Journal of Immunology (Baltimore, Md.: 1950), 195(10), 5035‑5044. https://doi.org/10.4049/jimmunol.1500429
  • Ménoret, S., De Cian, A., Tesson, L., Remy, S., Usal, C., Boulé, J.-B., … Anegon, I. (2015). Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins. Scientific Reports, 5, 14410. https://doi.org/10.1038/srep14410
  • Ménoret, S., Tesson, L., Remy, S., Usal, C., Ouisse, L.-H., Brusselle, L., … Anegon, I. (2015). Transgenic animals and genetic engineering techniques. Nantes, France, 2-3 July, 2015. Transgenic Research, 24(6), 1079‑1085. https://doi.org/10.1007/s11248-015-9904-6
  • Ranchoux, B., Antigny, F., Rucker-Martin, C., Hautefort, A., Péchoux, C., Bogaard, H. J., … Perros, F. (2015). Endothelial-to-mesenchymal transition in pulmonary hypertension. Circulation, 131(11), 1006‑1018. https://doi.org/10.1161/CIRCULATIONAHA.114.008750

2014

  •    Brüggemann, M., Osborn, M. J., Ma, B., Avis, S., Anegon, I., & Buelow, R. (2014). Transgenic Animals Derived by DNA Microinjection. In S. Dübel & J. M. Reichert (Éd.), Handbook of Therapeutic Antibodies (p. 77‑88). Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA. https://doi.org/10.1002/9783527682423.ch4
  • Larcher, T., Lafoux, A., Tesson, L., Remy, S., Thepenier, V., François, V., … Huchet, C. (2014). Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy. PloS One, 9(10), e110371. https://doi.org/10.1371/journal.pone.0110371
  • Ménoret, S., Tesson, L., Rémy, S., Usal, C., Thépenier, V., Thinard, R., … Anegon, I. (2014). Gene targeting in rats using transcription activator-like effector nucleases. Methods (San Diego, Calif.), 69(1), 102‑107. https://doi.org/10.1016/j.ymeth.2014.02.027
  • Ponce de León, V., Mérillat, A.-M., Tesson, L., Anegón, I., & Hummler, E. (2014). Generation of TALEN-mediated GRdim knock-in rats by homologous recombination. PloS One, 9(2), e88146. https://doi.org/10.1371/journal.pone.0088146
  • Remy, S., Tesson, L., Menoret, S., Usal, C., De Cian, A., Thepenier, V., … Anegon, I. (2014). Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases. Genome Research, 24(8), 1371‑1383. https://doi.org/10.1101/gr.171538.113

2013

  • Ma, B., Osborn, M. J., Avis, S., Ouisse, L.-H., Ménoret, S., Anegon, I., … Brüggemann, M. (2013). Human antibody expression in transgenic rats: comparison of chimeric IgH loci with human VH, D and JH but bearing different rat C-gene regions. Journal of Immunological Methods, 400401, 78‑86. https://doi.org/10.1016/j.jim.2013.10.007
  • Ménoret, S., Fontanière, S., Jantz, D., Tesson, L., Thinard, R., Rémy, S., … Anegon, I. (2013). Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology, 27(2), 703‑711. https://doi.org/10.1096/fj.12-219907
  • Ménoret, S., Tesson, L., Rémy, S., Thinard, R., Usal, C., Ouisse, L.-H., … Anegon, I. (2013). Technical advances in the generation of transgenic animals and in their applications. Nantes, France, June 7th 2013. Transgenic Research, 22(5), 1065‑1072. https://doi.org/10.1007/s11248-013-9736-1
  • Osborn, M. J., Ma, B., Avis, S., Binnie, A., Dilley, J., Yang, X., … Brüggemann, M. (2013). High-affinity IgG antibodies develop naturally in Ig-knockout rats carrying germline human IgH/Igκ/Igλ loci bearing the rat CH region. Journal of Immunology (Baltimore, Md.: 1950), 190(4), 1481‑1490. https://doi.org/10.4049/jimmunol.1203041

2012

  • Ménoret, S., Tesson, L., Remy, S., Usal, C., Iscache, A.-L., Thynard, R., … Anegon, I. (2012). Transgenesis and genome analysis, Nantes, France, June 6th 2011. Transgenic Research, 21(2), 449‑456. https://doi.org/10.1007/s11248-011-9541-7

2011

  • Iscache, A.-L., Ménoret, S., Tesson, L., Rémy, S., Usal, C., Pedros, C., … Anegon, I. (2011). Effects of BCL-2 over-expression on B cells in transgenic rats and rat hybridomas. International Immunology, 23(10), 625‑636. https://doi.org/10.1093/intimm/dxr071
  • Ménoret, S., Remy, S., Tesson, L., Usal, C., Iscache, A.-L., & Anegon, I. (2011). Generation of Transgenic Rats Using Microinjection of Plasmid DNA or Lentiviral Vectors. In S. Pease & T. L. Saunders (Éd.), Advanced Protocols for Animal Transgenesis: An ISTT Manual (p. 117‑135). Berlin, Heidelberg: Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-20792-1_7
  • Tesson, L., Usal, C., Ménoret, S., Leung, E., Niles, B. J., Remy, S., … Cost, G. J. (2011). Knockout rats generated by embryo microinjection of TALENs. Nature Biotechnology, 29(8), 695‑696. https://doi.org/10.1038/nbt.1940

2010

  • Geurts, A. M., Cost, G. J., Rémy, S., Cui, X., Tesson, L., Usal, C., … Buelow, R. (2010). Generation of gene-specific mutated rats using zinc-finger nucleases. Methods in Molecular Biology (Clifton, N.J.), 597, 211‑225. https://doi.org/10.1007/978-1-60327-389-3_15
  • Ménoret, S., Iscache, A.-L., Tesson, L., Rémy, S., Usal, C., Osborn, M. J., … Anegon, I. (2010). Characterization of immunoglobulin heavy chain knockout rats. European Journal of Immunology, 40(10), 2932‑2941. https://doi.org/10.1002/eji.201040939
  • Ménoret, S., Remy, S., Usal, C., Tesson, L., & Anegon, I. (2010). Generation of transgenic rats by microinjection of short DNA fragments. Methods in Molecular Biology (Clifton, N.J.), 597, 81‑92. https://doi.org/10.1007/978-1-60327-389-3_6
  • Ménoret, S., Tesson, L., Remy, S., Usal, C., Iscache, A.-L., & Anegon, I. (2010). “Transgenesis, recent technical developments and applications” Nantes, 8th June 2009. Transgenic Research, 19(4), 711‑714. https://doi.org/10.1007/s11248-009-9340-6
  • Rémy, S., Tesson, L., Ménoret, S., Usal, C., Scharenberg, A. M., & Anegon, I. (2010). Zinc-finger nucleases: a powerful tool for genetic engineering of animals. Transgenic Research, 19(3), 363‑371. https://doi.org/10.1007/s11248-009-9323-7
  • Remy, S., Tesson, L., Usal, C., Menoret, S., Bonnamain, V., Nerriere-Daguin, V., … Anegon, I. (2010). New lines of GFP transgenic rats relevant for regenerative medicine and gene therapy. Transgenic Research, 19(5), 745‑763. https://doi.org/10.1007/s11248-009-9352-2
  • Remy, Séverine, Nguyen, T. H., Ménoret, S., Tesson, L., Usal, C., & Anegon, I. (2010). The use of lentiviral vectors to obtain transgenic rats. Methods in Molecular Biology (Clifton, N.J.), 597, 109‑125. https://doi.org/10.1007/978-1-60327-389-3_8
  • Tesson, L., Rémy, S., Ménoret, S., Usal, C., & Anegon, I. (2010). Analysis by quantitative PCR of zygosity in genetically modified organisms. Methods in Molecular Biology (Clifton, N.J.), 597, 277‑285. https://doi.org/10.1007/978-1-60327-389-3_19

2009

  • Cozzi, J., Anegon, I., Braun, V., Gross, A.-C., Merrouche, C., & Cherifi, Y. (2009). Pronuclear DNA injection for the production of transgenic rats. Methods in Molecular Biology (Clifton, N.J.), 561, 73‑88. https://doi.org/10.1007/978-1-60327-019-9_5
  • Geurts, A. M., Cost, G. J., Freyvert, Y., Zeitler, B., Miller, J. C., Choi, V. M., … Buelow, R. (2009). Knockout rats via embryo microinjection of zinc-finger nucleases. Science (New York, N.Y.), 325(5939), 433. https://doi.org/10.1126/science.1172447
  • Tomat, S., Russo, F. P., Guido, M., Kechagias, G., Masier, A., Calore, C., … Burra, P. (2009). Study of the microcirculation in hDAF transgenic rat livers xenoperfused with human blood. Xenotransplantation, 16(2), 83‑90. https://doi.org/10.1111/j.1399-3089.2009.00519.x

2005

  • Tesson, L., Cozzi, J., Ménoret, S., Rémy, S., Usal, C., Fraichard, A., & Anegon, I. (2005). Transgenic Modifications of the Rat Genome. Transgenic Research, 14(5), 531–546. https://doi.org/10.1007/s11248-005-5077-z
  • Martin, C., Plat, M., Nerriére-Daguin, V., Coulon, F., Uzbekova, S., Venturi, E., Condé, F., Hermel, J.-M., Hantraye, P., Tesson, L., Anegon, I., Melchior, B., Peschanski, M., Le Mauff, B., Boeffard, F., Sergent-Tanguy, S., Neveu, I., Naveilhan, P., Soulillou, J.-P., … Vanhove, B. (2005). Transgenic expression of CTLA4-Ig by fetal pig neurons for xenotransplantation. Transgenic Research, 14(4), 373–384.
  • Toumaniantz, G., Seze, C., Serpillon, S., Menoret, S., Tesson, L., Anégone, I., & Gauthier, C. (2005). [Vascular beta-adrenergic remodeling in rat transgenic model over-expressing endothelial beta3-adrenoceptors]. Archives Des Maladies Du Coeur Et Des Vaisseaux, 98(7–8), 836–840.
  • Toumaniantz, G., Seze, C., Serpillon, S., Menoret, S., Tesson, L., Anégone, I., & Gauthier, C. (2005). [Vascular beta-adrenergic remodeling in rat transgenic model over-expressing endothelial beta3-adrenoceptors]. Archives Des Maladies Du Coeur Et Des Vaisseaux, 98(7–8), 836–840.

2002-2003

  • Braudeau, C., Bouchet, D., Toquet, C., Tesson, L., Ménoret, S., Iyer, S., Laboisse, C., Willis, D., Jarry, A., Buelow, R., Anegon, I., & Chauveau, C. (2003). Generation of heme oxygenase-1-transgenic rats. Experimental Biology and Medicine (Maywood, N.J.), 228(5), 466–471.
  • Stockmann, H., Verbakel, C., Okkema, P., Bonthuis, F., Menoret, S., Anegon, I., Marquet, R., & IJzermans, J. (2002). No functional benefit for hDAF-transgenic rat livers despite protection from tissue damage following perfusion with human serum. Transplant International: Official Journal of the European Society for Organ Transplantation, 15(12), 595–601. https://doi.org/10.1007/s00147-002-0463-4
  • Bouchet, D., Tesson, L., Ménoret, S., Charreau, B., Mathieu, P., Yagita, H., Duisit, G., & Anegon, I. (2002). Differential sensitivity of endothelial cells of various species to apoptosis induced by gene transfer of Fas ligand: Role of FLIP levels. Molecular Medicine (Cambridge, Mass.), 8(10), 612–623.
  • Ménoret, S., Aubert, D., Tesson, L., Braudeau, C., Pichard, V., Ferry, N., & Anegon, I. (2002). lacZ transgenic rats tolerant for beta-galactosidase: Recipients for gene transfer studies using lacZ as a reporter gene. Human Gene Therapy, 13(11), 1383–1390. https://doi.org/10.1089/104303402760128603
  • Aubert, D., Ménoret, S., Chiari, E., Pichard, V., Durand, S., Tesson, L., Moullier, P., Anegon, I., & Ferry, N. (2002). Cytotoxic immune response blunts long-term transgene expression after efficient retroviral-mediated hepatic gene transfer in rat. Molecular Therapy: The Journal of the American Society of Gene Therapy, 5(4), 388–396. https://doi.org/10.1006/mthe.2002.0561
  • Tesson, L., Heslan, J.-M., Ménoret, S., & Anegon, I. (2002). Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR. Transgenic Research, 11(1), 43–48.

1999-2001

  • Verbakel, C. A., Anegon, I., Ménoret, S., Marquet, R. L., & Ijzermans, J. N. (2001). Cellular immunity overrules the protective effect of human DAF as demonstrated in an ex vivo heart perfusion model. Transplantation Proceedings, 33(1–2), 781–782.
  • Ménoret, S., Jean, M., Tesson, L., Soulillou, J.-P., Anegon, I., & Charreau, B. (2001). Cryopreservation procedure for 1-cell and two-cell stage transgenic rat embryos. Transgenics, 3, 237–242.
  • Charreau, B., Ménoret, S., Tesson, L., Azimzadeh, A., Audet, M., Wolf, P., Marquet, R., Verbakel, C., Ijzermans, J., Cowan, P., Pearse, M., d’Apice, A., Soulillou, J. P., & Anegon, I. (1999). Protection against hyperacute xenograft rejection of transgenic rat hearts expressing human decay accelerating factor (DAF) transplanted into primates. Molecular Medicine (Cambridge, Mass.), 5(9), 617–630.
  • Menoret, S., Jean, M., Tesson, L., Soulillou, J., Anegon, I., & Charreau, B. (1999). Optimization of cryopreservation procedures for rat embryos. Transplantation Proceedings, 31(3), 1531–1532.
  • Tesson, L., Charreau, B., Ménoret, S., Gilbert, E., Soulillou, J. P., & Anegon, I. (1999). Endothelial expression of Fas ligand in transgenic rats under the temporal control of a tetracycline-inducible system. Transplantation Proceedings, 31(3), 1533–1534.

1996-1997

  • Charreau, B., Tesson, L., Menoret, S., Buscail, J., Soulillou, J. P., & Anegon, I. (1997). Production of transgenic rats for human regulators of complement activation. Transplantation Proceedings, 29(3), 1770.
  • Charreau, B., Ménoret, S., Tesson, L., Soulillou, J.-P., & Anegon, I. (1997). Generation and use of transgenic rats. Rat Genome, 3, 125–132.
  • Charreau, B., Tesson, L., Buscail, J., Soulillou, J. P., & Anegon, I. (1996). Analysis of human CD59 tissue expression directed by the CMV-IE-1 promoter in transgenic rats. Transgenic Research, 5(6), 443–450.
  • Charreau, B., Tesson, L., Soulillou, J. P., Pourcel, C., & Anegon, I. (1996). Transgenesis in rats: Technical aspects and models. Transgenic Research, 5(4), 223–234.